dermal fibroblast cell line hdfa  (ATCC)

Journal: Cell Death & Disease

Article Title: Identification of a small molecule targeting EPLIN as a novel strategy for the treatment of pediatric neuroblastoma and medulloblastoma

doi: 10.1038/s41419-025-07876-7

Figure Lengend Snippet: A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).

Article Snippet: hTERT-immortalized retinal pigment epithelial cell line (Cat. #CRL-4000) and neuroblastoma cell lines SK-N-AS (Cat. #CRL-2137), SH-SY5Y (Cat. #CRL-2266), SK-N-SH, (Cat. #HTB-11), CHP-212 (Cat. #CRL-2137), IMR-32 (Cat. #CRL-127), SK-N-BE2 (Cat. #CRL-2271) and medulloblastoma cell lines HDFa (Cat. #PCS-201-012), D283 (Cat. #HTB-185) and Daoy (Cat. #HTB-186) was obtained from (ATCC, Manassas, VA, USA). hTERT, SK-N-AS, SH-SY5Y, SK-N-SH, CHP-212, IMR-32, SK-N-BE [ ] was maintained in 1:1 ratio of EMEM (ATCC, Cat. #30-2003) and Ham’s F-12 Nutrient Mix (Thermo Fisher Scientific, Waltham, MA, USA, Cat. #11765054) supplemented with 1% penicillin streptomycin (Thermo Fisher Scientific, Cat. #15140-122) and 10% FBS (Thermo Fisher Scientific, Cat. #10270-106).

Techniques: Drug discovery, Over Expression, Derivative Assay, Control, Colony Assay, Cell Culture

Journal: Cancer Management and Research

Article Title: Potential of Using New Indole- and Benzimidazo[1,2-C]quinazolines in Anticancer Therapy Based on Mesenchymal Stem Cells

doi: 10.2147/CMAR.S516593

Figure Lengend Snippet: Evaluation of the IC 50 values of compound A determined for ASC52-telo cells and HDFa cells in 48- and 72-hour cell cultures.

Article Snippet: Human skin fibroblast cell line (HDFa) (ATCC, PCS-201-012, Primary Dermal Fibroblast; Normal, Human, Adult) - a normal, adherent, skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery and skin diseases including scleroderma.

Techniques:

Journal: Cancer Management and Research

Article Title: Potential of Using New Indole- and Benzimidazo[1,2-C]quinazolines in Anticancer Therapy Based on Mesenchymal Stem Cells

doi: 10.2147/CMAR.S516593

Figure Lengend Snippet: Evaluation of the IC 50 values of compound B determined for ASC52-telo cells and HDFa cells in 48- and 72-hour cell cultures.

Article Snippet: Human skin fibroblast cell line (HDFa) (ATCC, PCS-201-012, Primary Dermal Fibroblast; Normal, Human, Adult) - a normal, adherent, skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery and skin diseases including scleroderma.

Techniques:

Journal: Protoplasma

Article Title: Morphological, anatomical, and bioactive properties of Hypericum scabrum L.: effects on diabetes mellitus, Alzheimer’s disease, and HDFa fibroblasts and U87-MG cancer cells

doi: 10.1007/s00709-025-02037-1

Figure Lengend Snippet: Cytotoxicity and anticancer analyses of H. scabrum L. CH 2 Cl 2 isolate on human fibroblast (HDFa) cell line and human glioblastoma (U87MG) cell culture for 24 h of application

Article Snippet: The HDFa human fibroblast cell line (ATCC® PCS-201–012TM) was cultured to full confluence in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1% penicillin/streptomycin antibiotics and 10% fetal bovine serum (FBS).

Techniques: Cell Culture

Journal: Protoplasma

Article Title: Morphological, anatomical, and bioactive properties of Hypericum scabrum L.: effects on diabetes mellitus, Alzheimer’s disease, and HDFa fibroblasts and U87-MG cancer cells

doi: 10.1007/s00709-025-02037-1

Figure Lengend Snippet: Cytotoxicity and anticancer analyses of H. scabrum L. dH 2 O isolate on human fibroblast (HDFa) cell line and human glioblastoma (U87MG) cell culture for 24 h of application

Article Snippet: The HDFa human fibroblast cell line (ATCC® PCS-201–012TM) was cultured to full confluence in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1% penicillin/streptomycin antibiotics and 10% fetal bovine serum (FBS).

Techniques: Cell Culture

Journal: Protoplasma

Article Title: Morphological, anatomical, and bioactive properties of Hypericum scabrum L.: effects on diabetes mellitus, Alzheimer’s disease, and HDFa fibroblasts and U87-MG cancer cells

doi: 10.1007/s00709-025-02037-1

Figure Lengend Snippet: Cytotoxicity and anticancer analyses of H. scabrum L. EtOAc isolate on human fibroblast (HDFa) cell line and human glioblastoma (U87MG) cell culture for 24 h of application

Article Snippet: The HDFa human fibroblast cell line (ATCC® PCS-201–012TM) was cultured to full confluence in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1% penicillin/streptomycin antibiotics and 10% fetal bovine serum (FBS).

Techniques: Cell Culture

Journal: Protoplasma

Article Title: Morphological, anatomical, and bioactive properties of Hypericum scabrum L.: effects on diabetes mellitus, Alzheimer’s disease, and HDFa fibroblasts and U87-MG cancer cells

doi: 10.1007/s00709-025-02037-1

Figure Lengend Snippet: Cytotoxicity and anticancer analyses of H. scabrum L. MeOH isolate on human fibroblast (HDFa) cell line and human glioblastoma (U87MG) cell culture for 24 h of application

Article Snippet: The HDFa human fibroblast cell line (ATCC® PCS-201–012TM) was cultured to full confluence in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1% penicillin/streptomycin antibiotics and 10% fetal bovine serum (FBS).

Techniques: Cell Culture

Journal: Antioxidants

Article Title: Synergistic Anti-Cancer Effects of Curcumin and Thymoquinone Against Melanoma

doi: 10.3390/antiox13121573

Figure Lengend Snippet: Effect of thymoquinone, curcumin, and their combination on the viability of human (A375) metastatic melanoma cells and the two healthy human keratinocyte (HaCaT) and human dermal fibroblast (HDfa) cell lines. The values are presented as the percentage of cell death. The data represent the mean ± SD of three independent experiments conducted in triplicate (vs. control, * p < 0.001).

Article Snippet: The A375 human melanoma cell lines, primary human dermal fibroblast adult (HDFa) cell lines, and HaCaT human keratinocytes cell lines were purchased from the ATCC, VA, USA, and AddexBio, San Diego, CA, USA.

Techniques: Control